Biomedical and Biological Engineering | Article | Published 2016-06-01

Characterization of Small RNAs and Their Targets from Fusarium oxysporum Infected and Noninfected Cotton Root Tissues

Authors:

E. J. Devor

Umid Shapulatov

Zabardast T Buriev

Mauricio Ulloa

Sukumar Saha

Tohir Norov

Shukhrat E Shermatov

A Abdukarimov

Johnie N Jenkins

Publisher: Springer US
Collection: Plant Molecular Biology Reporter
Keywords: Genomics and bioinformatics

Abstract

Genes for host-plant resistant do exist in cotton (Gossypium spp.) but improvement of cotton cultivars with resistance is difficult due to intensive breeding. Identifying molecular-genetic mechanisms associated with disease resistance can offer a new way to combat a serious threat such as Fusarium oxysporum f. sp. vasinfectum (FOV). Here, we captured and annotated “top-layer” of abundantly and specifically expressed cotton root small RNA (sRNA) including microRNA (miR) sequences during FOV pathogenesis using size-directed and adenylated linker-based sRNA cloning strategy. A total of 4116 candidate sRNA sequences with 16 to 30 nucleotide (nt) length were identified from four complementary DNA (cDNA) libraries of noninfected and FOV race 3-infected roots of susceptible (“11970”) versus resistant (“Mebane B-1”) cotton genotypes (G. hirsutum L.). The highest numbers of sRNA signatures were …

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