Biomedical and Biological Engineering | Article | Published 2012

Solid-phase colorimetric method for the quantification of fucoidan

Authors:

Jung Min Lee

Z-U Shin

Gafurjon T Mavlonov

Tae-Hoo Yi

Publisher: Springer-Verlag
Collection: Applied biochemistry and biotechnology
Keywords: Genomics and bioinformatics

Abstract

We described the simple, selective, and rapid method for determination of fucoidans using methylene blue staining of sulfated polysaccharides, immobilized into filter paper and consequent optic density (at A 663 nm) measurement of the eluted dye from filter paper. This solid-phase method allows selective determination of 1–20 μg fucoidan in presence of potentially interfering compounds (alginic acid, DNA, salts, proteins, and detergents). Further, we demonstrated the alternative way of using image processing software for fucoidan quantification without extraction of methylene blue dye from stained spots of fucoidan–dye complex.

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