Biomedical and Biological Engineering | Article | Published 2015-04-29

Molecular confirmation of Gossypium hirsutum chromosome substitution lines

Authors:

David M

Abdusalom K Makamov

Dwaine Raska

Osman A Gutiérrez

Shivapriya Manchali

Dewayne Deng

Sukumar Saha

Johnie N Jenkins

Publisher: Springer Netherlands
Collection: Euphytica
Keywords: Genomics and bioinformatics

Abstract

The primary gene pool for tetraploid cotton species includes Gossypium hirsutum L., as well as the other four 2n = 52 species of Gossypium (G. barbadense, G. mustellinum, G. tomentosum and G. darwinii). To help overcome barriers to effective introgression, we have developed a number of alien chromosome substitution (CS) lines from G. barbadense, G. mustellinum and G. tomentosum, most of which are nearly isogenic to the inbred ‘Texas Marker-1’, a genetic standard. At the time CS line development was initiated, molecular markers did not exist for some CS lines, and most of these lines were developed based on cytological analysis without using any marker-based testing. Here we report on tests with SSR markers from one ore more linkage maps specific to the substituted chromosome or chromosome segment from one or more linkage maps to assess the constitution and genetic identity of the CS …

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