Agricultural and Biosystems Engineering | Conference paper | Published 2023

SIZE-EXCLUSION CHROMATOGRAPHY OF PULLULANS AND ARABINOGALACTANS

Authors:

Ziyovadin Kamolov

Collection: «TA'LIM, FAN VA ISHLAB CHIQARISHNING DOLZARB MUAMMOLARI» MAVZUSIDAGI RESPUBLIKA ILMIY AMALIY KONFERENSIYA PLAMI
Keywords: Size-exclusion chromatography, PL Aquagel-OH Mixed, Elution volume, hydrodynamic volume

Abstract

The validity of the Universal calibration concept in aqueous Sizeexclusion chromatography for arabinogalactan and pullulans is presented. The electrostatic effects of these polysaccharides during separation process were eliminated by utilizing of a water-salt eluent as a mobile phase, and the sizeexclusion separation mechanism of this method was realized.

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